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Characterization and online detection of aromatic alkaloids in the ascidian Lissoclinum cf. badium by liquid chromatography/UV detection mass spectrometry
Author(s) -
Liu Hongwei,
Gao Hao,
Namikoshi Michio,
Kobayashi Hisayoshi,
Mangindaan Remy E. P.,
Wang Naili,
Yao Xinsheng
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2833
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray ionization , high performance liquid chromatography , tandem mass spectrometry , moiety , fragmentation (computing) , ion trap , organic chemistry , computer science , operating system
A detection method based on high‐performance liquid chromatography coupled with ultraviolet diode‐array detection and electrospray ionization ion trap tandem mass spectrometry (HPLC‐UV‐ESI‐MS/MS) was developed to investigate the total alkaloids prepared from the ascidian Lissoclinum cf. badium . The aromatic alkaloids possessing polysulfide structures are the major bioactive constituents isolated from ascidians of the genera Lissoclinum , Eudistoma , and Polycitor . These compounds presented various important biological activities. The ESI‐MS fragmentation behavior of this kind of alkaloids was studied, and the fragmentation was characterized by elimination of the NH(CH 3 ) 2 moiety. The use of reversed‐phase HPLC/UV‐ESI‐MS allowed the online separation and detection of 25 aromatic alkaloids. This approach provided data that can be used for detection of biologically active aromatic alkaloids from marine organisms. Copyright © 2006 John Wiley & Sons, Ltd.