Characterization and online detection of aromatic alkaloids in the ascidian  Lissoclinum  cf.  badium  by liquid chromatography/UV detection mass spectrometry | Zendy

Liu Hongwei | Zendy; Gao Hao | Zendy; Namikoshi Michio | Zendy; Kobayashi Hisayoshi | Zendy; Mangindaan Remy E. P. | Zendy; Wang Naili | Zendy; Yao Xinsheng | Zendy

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Characterization and online detection of aromatic alkaloids in the ascidian Lissoclinum cf. badium by liquid chromatography/UV detection mass spectrometry

Author(s) -

Liu Hongwei,

Gao Hao,

Namikoshi Michio,

Kobayashi Hisayoshi,

Mangindaan Remy E. P.,

Wang Naili,

Yao Xinsheng

Publication year - 2006

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.2833

Subject(s) - chemistry , chromatography , mass spectrometry , electrospray ionization , high performance liquid chromatography , tandem mass spectrometry , moiety , fragmentation (computing) , ion trap , organic chemistry , computer science , operating system

A detection method based on high‐performance liquid chromatography coupled with ultraviolet diode‐array detection and electrospray ionization ion trap tandem mass spectrometry (HPLC‐UV‐ESI‐MS/MS) was developed to investigate the total alkaloids prepared from the ascidian Lissoclinum cf. badium . The aromatic alkaloids possessing polysulfide structures are the major bioactive constituents isolated from ascidians of the genera Lissoclinum , Eudistoma , and Polycitor . These compounds presented various important biological activities. The ESI‐MS fragmentation behavior of this kind of alkaloids was studied, and the fragmentation was characterized by elimination of the NH(CH 3 ) 2 moiety. The use of reversed‐phase HPLC/UV‐ESI‐MS allowed the online separation and detection of 25 aromatic alkaloids. This approach provided data that can be used for detection of biologically active aromatic alkaloids from marine organisms. Copyright © 2006 John Wiley & Sons, Ltd.

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