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Liquid chromatography/mass spectrometry analysis of PHY906, a Chinese medicine formulation for cancer therapy
Author(s) -
Ye Min,
Liu ShwuHuey,
Jiang Zaoli,
Lee Yashang,
Tilton Robert,
Cheng YungChi
Publication year - 2007
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2832
Subject(s) - chemistry , chromatography , formic acid , mass spectrometry , electrospray ionization , triterpene , mass spectrum , glycoside , acetonitrile , organic chemistry , medicine , alternative medicine , pathology
PHY906 is a Chinese medicine formulation prepared from four medicinal herbs for adjuvant cancer chemotherapy. In this paper, liquid chromatography/electrospray ionization time‐of‐flight mass spectrometry (LC/ESI‐TOFMS) was used to clarify the chemical composition of PHY906. The aqueous extract of PHY906 was separated on a Waters Atlantis C 18 column, and was eluted with acetonitrile/0.05% (v/v) formic acid. The separated compounds were identified with pure standards, or tentatively characterized by analyzing their mass spectra recorded in both negative and positive ion polarity modes. Further structural information was obtained from in‐source fragmentation. Based on the LC/MS analysis, we proposed the structures for 64 bioactive compounds, including flavonoids, triterpene saponins, and monoterpene glycosides. All the compounds identified from PHY906 were further assigned in the four individual herbs, and some of them are reported for the first time. Copyright © 2007 John Wiley & Sons, Ltd.