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Development of a liquid chromatography/electrospray ionization tandem mass spectrometric method for the determination of hydroxyl radical
Author(s) -
Yang Fen,
Zhang Ruiping,
He Jiuming,
Abliz Zeper
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2815
Subject(s) - chemistry , chromatography , electrospray ionization , electrospray , tandem mass spectrometry , selected reaction monitoring , tandem , hydroxyl radical , liquid chromatography–mass spectrometry , relative standard deviation , mass spectrometry , analytical chemistry (journal) , radical , organic chemistry , detection limit , materials science , composite material
A rapid method combining liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) was developed for the determination of the hydroxyl radical ( · OH). · OH generated via Fenton reaction was spin‐trapped by 5,5‐dimethyl‐1‐pyrroline N ‐oxide (DMPO) and then analyzed by LC/ESI‐MS/MS in multiple reaction monitoring (MRM) mode, using N ‐methyl‐2‐pyrrolidone (NMP) as the internal standard. The peak area ratio of DMPO‐OH to NMP positively correlated with the concentration of · OH. The relative standard deviation (RSD) of the method was 1.13% (n = 8). The present method was successfully applied to evaluate the · OH scavenging capacity of several phenolic acids. Copyright © 2006 John Wiley & Sons, Ltd.