Premium
Analysis of succinylacetone, as a Girard T derivative, in urine and dried bloodspots by flow injection electrospray ionization tandem mass spectrometry
Author(s) -
Johnson David W.,
Gerace Rosemarie,
Ranieri Enzo,
Trinh MinhUyen,
Fingerhut Ralph
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2806
Subject(s) - chemistry , electrospray ionization , urine , chromatography , tandem mass spectrometry , mass spectrometry , derivative (finance) , tyrosinemia , electrospray , tyrosine , biochemistry , financial economics , economics
Flow injection electrospray ionization tandem mass spectrometric methods for succinylacetone (SA) in 250 µL urine, using d 5 ‐SA as internal standard, and in 3 mm dried bloodspots, using 13 C 4 ‐SA as internal standard, are described. Selectivity and sensitivity of analysis is achieved by the use of a mono‐Girard T derivative. Measured SA infant urine normal range (n = 20) is 0.013–0.27 µmol/mmol creatinine. Measured SA newborn bloodspot normal range (n = 152) is 0–0.30 µmol/L. Bloodspots from children with hepatorenal tyrosinemia type 1, and kept at room temperature for up to 7 years, afforded SA concentrations of 0.9–5.7 µmol/L. Copyright © 2006 John Wiley & Sons, Ltd.