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Quantification of trandolapril and its metabolite trandolaprilat in human plasma by liquid chromatography/tandem mass spectrometry using solid‐phase extraction
Author(s) -
Nirogi Ramakrishna V. S.,
Kandikere Vishwottam N.,
Shrivastava Wishu,
Mudigonda Koteshwara
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2794
Subject(s) - chemistry , trandolapril , chromatography , solid phase extraction , metabolite , tandem mass spectrometry , liquid chromatography–mass spectrometry , extraction (chemistry) , detection limit , selected reaction monitoring , mass spectrometry , electrospray ionization , analyte , analytical chemistry (journal) , angiotensin converting enzyme , ace inhibitor , medicine , biochemistry , blood pressure , radiology
A sensitive high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous quantification of trandolapril and its metabolite trandolaprilat in human plasma using ramipril as an internal standard. Following solid‐phase extraction, the analytes were separated using an isocratic mobile phase on a reversed‐phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M–H] − ions, m/z 429/168 for trandolapril, m/z 401/168 for trandolaprilat and m/z 415/166 for the internal standard. The method exhibited a linear dynamic range of 20–10 000 pg/mL for both trandolapril and trandolaprilat in human plasma. The lower limit of quantification was 20 pg/mL for both trandolapril and its metabolite. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2006 John Wiley & Sons, Ltd.