Liquid chromatography/tandem mass spectrometry assay for the quantification of troxerutin in human plasma

A simple, rapid, sensitive and specific liquid chromatography/tandem mass spectrometry method was developed and validated to quantify troxerutin in human plasma. The analyte and rutin, used as the internal standard, were analyzed on a Phenomenex Synergi Fusion RP column interfaced with a triple‐quadrupole tandem mass spectrometer using positive electrospray ionization. Acetonitrile/water (20:80 v/v) was used as the isocratic mobile phase, with 0.1% formic acid in water. A simple sample preparation method of protein precipitation with perchloric acid was employed. The assay was linear over the concentration range 31.25–4000 pg/mL. Correlation coefficients generated by linear regression with a 1/ x 2 weighting factor ranged from 0.9991 to 0.9996. The intra‐ and inter‐day precision over the entire concentration range were less than 12.28%. The method was successfully applied to a pharmacokinetic study after oral administration of a 300 mg troxerutin drop pill to 18 healthy volunteers. Copyright © 2006 John Wiley & Sons, Ltd.