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Liquid chromatography/tandem mass spectrometry assay for the quantification of troxerutin in human plasma
Author(s) -
Liu Fei,
Xu Yu,
Rui Lei,
Gao Shu,
Dong Haijun,
Guo Qingxiang
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2764
Subject(s) - chemistry , chromatography , protein precipitation , formic acid , mass spectrometry , electrospray ionization , triple quadrupole mass spectrometer , selected reaction monitoring , tandem mass spectrometry , analyte , liquid chromatography–mass spectrometry
A simple, rapid, sensitive and specific liquid chromatography/tandem mass spectrometry method was developed and validated to quantify troxerutin in human plasma. The analyte and rutin, used as the internal standard, were analyzed on a Phenomenex Synergi Fusion RP column interfaced with a triple‐quadrupole tandem mass spectrometer using positive electrospray ionization. Acetonitrile/water (20:80 v/v) was used as the isocratic mobile phase, with 0.1% formic acid in water. A simple sample preparation method of protein precipitation with perchloric acid was employed. The assay was linear over the concentration range 31.25–4000 pg/mL. Correlation coefficients generated by linear regression with a 1/ x 2 weighting factor ranged from 0.9991 to 0.9996. The intra‐ and inter‐day precision over the entire concentration range were less than 12.28%. The method was successfully applied to a pharmacokinetic study after oral administration of a 300 mg troxerutin drop pill to 18 healthy volunteers. Copyright © 2006 John Wiley & Sons, Ltd.