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Direct high‐resolution peptide and protein analysis by desorption electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Author(s) -
Bereman Michael S.,
Nyadong Leonard,
Fernandez Facundo M.,
Muddiman David C.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2759
Subject(s) - fourier transform ion cyclotron resonance , chemistry , mass spectrometry , ion cyclotron resonance , protein mass spectrometry , analytical chemistry (journal) , electrospray ionization , ion source , desorption electrospray ionization , top down proteomics , resolution (logic) , ionization , maldi imaging , selected ion monitoring , sample preparation in mass spectrometry , ion , desorption , chromatography , matrix assisted laser desorption/ionization , cyclotron , thermal ionization mass spectrometry , adsorption , organic chemistry , gas chromatography–mass spectrometry , artificial intelligence , computer science
We report the first coupling of a desorption electrospray ionization (DESI) ion source to Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FT‐ICR‐MS) for high‐resolution protein analysis. The DESI FT‐ICR‐MS source design is described in detail along with preliminary data obtained on peptides and proteins ranging from 1 to 5.7 kDa. Copyright © 2006 John Wiley & Sons, Ltd.