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The effect of the source pressure on the abundance of ions of noncovalent protein assemblies in an electrospray ionization orthogonal time‐of‐flight instrument
Author(s) -
Tahallah Nora,
Pinkse Martijn,
Maier Claudia S.,
Heck Albert J. R.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.275
Subject(s) - chemistry , electrospray ionization , ion , electrospray , mass spectrometry , ionization , analytical chemistry (journal) , non covalent interactions , abundance (ecology) , mass spectrum , phase (matter) , ion source , chromatography , molecule , organic chemistry , biology , hydrogen bond , fishery
The effect of elevating the pressure in the interface region of an electrospray ionization orthogonal time‐of‐flight mass spectrometer on the ion intensity of different noncovalent protein assemblies has been investigated. Elevating the pressure in the interface region generally led to an enhanced detection of high m/z ions. The optimum pressure was found to be dependent on the m/z value of the ions. This pressure effect should be carefully addressed when relating ion abundance in the mass spectra to solution phase abundance of noncovalent protein assemblies. Copyright © 2001 John Wiley & Sons, Ltd.