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The molecular scanner in microscope mode
Author(s) -
Luxembourg Stefan L.,
Vaezaddeh Ali R.,
Amstalden Erika R.,
ZimmermannIvol Catherine G.,
Hochstrasser Denis F.,
Heeren Ron M.A.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2747
Subject(s) - chemistry , microscope , mass spectrometry , analytical chemistry (journal) , scanner , microprobe , maldi imaging , mass spectrometry imaging , resolution (logic) , mass spectrum , chromatography , matrix assisted laser desorption/ionization , desorption , optics , artificial intelligence , adsorption , mineralogy , physics , organic chemistry , computer science
The combination of microscope mode matrix‐assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) with protein identification methodology: the molecular scanner, was explored. The molecular scanner approach provides improvement of sensitivity of detection and identification of high‐mass proteins in microscope mode IMS. The methodology was tested on protein distributions obtained after separation by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS‐PAGE). High‐quality, high‐spatial‐resolution ion images were recorded on a TRIFT‐II ion microscope after gold coating of the MALDI sample preparation on the poly(vinylidenedifluoride) capture membranes. The sensitivity of the combined method is estimated to be 5 pmol. The minimum amount of sample consumed, needed for identification, was estimated to be better than 100 fmol. Software tools were developed to analyze the spectral data and to generate broad mass range and single molecular component microscope mode ion images and single mass‐to‐charge ratio microprobe mode images. Copyright © 2006 John Wiley & Sons, Ltd.