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A metabonomic analysis of plasma from Zucker rat strains using gas chromatography/mass spectrometry and pattern recognition
Author(s) -
Major Hilary J.,
Williams Rebecca,
Wilson Amy J.,
Wilson Ian D.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2732
Subject(s) - chemistry , chromatography , metabolite , mass spectrometry , metabolomics , gas chromatography–mass spectrometry , gas chromatography , protein precipitation , biochemistry
Plasma obtained from three strains of Zucker rats was analysed using capillary gas chromatography/mass spectrometry (GC/MS) to obtain global metabolite profiles as part of a series of metabonomic investigations of animal models of diabetes. Samples were obtained from 20‐week‐old male wild‐type Zucker lean, (fa/fa) obese and lean/(fa) animals and were analysed following protein precipitation, using acetonitrile, and derivatisation. Subsequent data analysis using principal components analysis (PCA) and orthogonal projection to latent structures (OPLS) revealed differences between the plasma metabolite profiles of the three strains, with those of the Zucker lean and the lean/(fa) crosses being similar to each other whilst differing from the (fa/fa) obese strain. Copyright © 2006 John Wiley & Sons, Ltd.