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Accurate mass measurement of synthetic analogues of prazosine by matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry
Author(s) -
Andalò Christian,
Bocchini Paola,
Pozzi Romina,
Galletti Guido C.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.272
Subject(s) - chemistry , mass spectrometry , reflectron , time of flight mass spectrometry , analytical chemistry (journal) , resolution (logic) , mass spectrum , desorption , matrix (chemical analysis) , molecule , chromatography , matrix assisted laser desorption/ionization , surface enhanced laser desorption/ionization , mass , ionization , sample preparation in mass spectrometry , electrospray ionization , ion , organic chemistry , computer science , adsorption , artificial intelligence
Matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOFMS) has been investigated as a tool for accurate determination of the molecular mass of synthetic analogues of prazosine, a molecule used for the treatment of hypertension. Samples were dissolved in methanol, mixed with mass calibration standards, and crystallised on the target with α‐cyano‐4‐hydroxycinnamic acid as matrix. Acquisition of spectra was rapidly completed in reflectron mode, allowing high resolution (6000–10000) and sensitive (about 1–10 pmol of sample on target) determination of the synthetic products. The results show the effectiveness of MALDI‐TOFMS for accurate mass determination of these fairly large molecules, which are otherwise difficult to analyse by other high‐resolution mass spectrometric techniques. Copyright © 2001 John Wiley & Sons, Ltd.