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Quantitative analyses of indoloquinazoline alkaloids in Fructus Evodiae by high‐performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry
Author(s) -
Zhou Yan,
Li ShengHong,
Jiang RenWang,
Cai Min,
Liu Xin,
Ding LiSheng,
Xu HongXi,
But Paul PuiHay,
Shaw PangChui
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2705
Subject(s) - rutaecarpine , evodiamine , chemistry , chromatography , atmospheric pressure chemical ionization , repeatability , tandem mass spectrometry , analyte , mass spectrometry , high performance liquid chromatography , liquid chromatography–mass spectrometry , quantitative analysis (chemistry) , selected reaction monitoring , chemical ionization , ionization , ion , organic chemistry
Fructus Evodiae (Wuzhuyu), the fruits of Evodia rutaecarpa and related varieties, is widely used in traditional Chinese medicine. The bioactive constituents include the indoloquinazoline alkaloids rutaecarpine, evodiamine and dehydroevodiamine. A new assay based on high‐performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/UV/APCI‐MS/MS) was developed for the measurement of the indoloquinazoline alkaloids in commercial Fructus Evodiae products. Initially, the MS/MS fragmentation pathways of indoloquinazoline alkaloids were investigated to identify fragment ions that might be useful for the sensitive and selective detection of trace indoloquinazoline alkaloids during LC/MS/MS. Then, quantitative MS analysis of five indoloquinazoline alkaloids in 12 commercial Fructus Evodiae products from different geographical sources was performed. Analyte recovery was in the range of 97.5–105.3% for all with relative standard deviations (RSDs) below 6%, the intra‐assay and inter‐assay RSDs were less than 7%, and good linear relationships were shown with correlation coefficients for the analytes exceeding 0.999. Therefore, this LC/MS/MS assay facilitated the rapid quantitative analysis of rutaecarpine, evodiamine, evodiamide, 14‐formyldihydrorutaecarpine and dehydroevodiamine in 12 commercial Fructus Evodiae products with excellent recovery, repeatability, accuracy and sensitivity. This method is simple and specific and can be used for identification and quality control of this traditional Chinese remedy. Copyright © 2006 John Wiley & Sons, Ltd.