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Application of liquid chromatography/ion trap mass spectrometry to the characterization of the related substances of clarithromycin
Author(s) -
Leonard Stefanie,
Ferraro Monica,
Adams Erwin,
Hoogmartens Jos,
Schepdael Ann Van
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2704
Subject(s) - chemistry , chromatography , mass spectrometry , characterization (materials science) , ion trap , trap (plumbing) , ion , clarithromycin , organic chemistry , nanotechnology , antibiotics , biochemistry , materials science , environmental engineering , engineering
A selective reversed‐phase liquid chromatography/mass spectrometry (LC/MS n ) method was developed for the characterization of components of the semi‐synthetic macrolide clarithromycin. Mass spectral data were acquired online on a LCQ ion trap mass spectrometer equipped with an electrospray ionization source operated in the positive ion mode. One unknown compound was structurally elucidated and two other unknowns were characterized using the MS/MS and MS n collision‐induced dissociation spectra of reference substances as interpretative templates, combined with knowledge of the nature of functional group fragmentation behaviour. Given the importance attached to the identification of impurities of unknown identity in pharmaceutical substances, this study is useful for companies producing clarithromycin. Copyright © 2006 John Wiley & Sons, Ltd.