Determination of aranidipine and its active metabolite in human plasma by liquid chromatography/negative electrospray ionization tandem mass spectrometry | Zendy

Tian Lei | Zendy; Jiang Juanjuan | Zendy; Huang Yiling | Zendy; Xu Li | Zendy; Liu Hong | Zendy; Li Yishi | Zendy

Premium

Determination of aranidipine and its active metabolite in human plasma by liquid chromatography/negative electrospray ionization tandem mass spectrometry

Author(s) -

Tian Lei,

Jiang Juanjuan,

Huang Yiling,

Xu Li,

Liu Hong,

Li Yishi

Publication year - 2006

Publication title -

rapid communications in mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.528

H-Index - 136

eISSN - 1097-0231

pISSN - 0951-4198

DOI - 10.1002/rcm.2663

Subject(s) - chemistry , chromatography , electrospray ionization , direct electron ionization liquid chromatography–mass spectrometry interface , mass spectrometry , metabolite , tandem mass spectrometry , liquid chromatography–mass spectrometry , extractive electrospray ionization , protein mass spectrometry , sample preparation in mass spectrometry , electrospray , selected reaction monitoring , human plasma , chemical ionization , ionization , ion , organic chemistry , biochemistry

A simple, sensitive and rapid high‐performance liquid chromatography/negative electrospray ionization tandem mass spectrometry method was developed and validated for the assay of aranidipine (AR) and its active metabolite (AR‐M) in human plasma. Following a liquid‐liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed‐phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [MH] − ions, m/z 387.0 → 164.0 for AR, m/z 389.1 → 208.1 for AR‐M and m/z 359.0 → 121.8 for the internal standard. The assay exhibited a linear dynamic range of 0.02–10 ng · mL −1 for AR and 0.2–100 ng · mL −1 for AR‐M in human plasma. The limits of quantitation were 0.02 ng · mL −1 for AR and 0.2 ng · mL −1 for AR‐M. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.8 min for each sample exhibited its high‐throughout analysis ability. The validated method can be applied to analyze human plasma samples for pharmacokinetic studies. Copyright © 2006 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research