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Surface‐activated chemical ionization and high‐flow gradient chromatography to reduce matrix effect
Author(s) -
Cristoni Simone,
Bernardi Luigi Rossi,
Gerthoux Piermario,
Mocarelli Paolo,
Brambilla Maura,
Gonella Elisabetta,
Guidugli Federico,
Cantu Marco
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2599
Subject(s) - chemistry , chromatography , atmospheric pressure chemical ionization , analyte , matrix (chemical analysis) , column chromatography , electrospray ionization , chemical ionization , elution , ionization , mass spectrometry , analytical chemistry (journal) , organic chemistry , ion
The new atmospheric pressure chemical ionization source, named surface‐activated chemical ionization (SACI), has been used in conjunction with high‐flow gradient chromatography to reduce the matrix effect. This high‐flow gradient chromatography approach avoids the co‐elution of analyte and biological matrix compounds that leads to a reduction in quantitation errors due to matrix effect. However, this approach cannot be employed with the classical electrospray ionization (ESI) source that usually works at low eluent flow (< 300 µL/min). SACI can work at high eluent flow (100–2000 µL/min) and can be employed in conjunction with high‐flow gradient chromatography. The reduction in matrix effect in tacrolimus analysis in protein‐precipitated blood samples, an important immunosuppressive agent for renal transplantation, is presented and discussed. Copyright © 2006 John Wiley & Sons, Ltd.