Premium
Quantification of tizanidine in human plasma by liquid chromatography coupled to tandem mass spectrometry
Author(s) -
Nirogi Ramakrishna V. S.,
Kandikere Vishwottam N.,
Shukla Manoj,
Mudigonda Koteshwara,
Maurya Santosh
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2592
Subject(s) - chemistry , chromatography , tizanidine , analyte , selected reaction monitoring , electrospray , human plasma , bioequivalence , liquid chromatography–mass spectrometry , tandem mass spectrometry , mass spectrometry , detection limit , bioavailability , extraction (chemistry) , medicine , bioinformatics , spasticity , biology , physical therapy
Abstract A simple, sensitive and rapid high‐performance liquid chromatography/positive ion electrospray tandem mass spectrometry (MS/MS) method was developed and validated for the assay of tizanidine in human plasma. Following liquid‐liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed‐phase column and analyzed by MS/MS in the selected reaction monitoring mode. The assay exhibited a linear dynamic range of 50–5000 pg/mL for tizanidine in human plasma. The lower limit of quantification was 50 pg/mL with a relative standard deviation of less than 13%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 300 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2006 John Wiley & Sons, Ltd.