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Simultaneous identification and verification of Bacillus anthracis
Author(s) -
Krishnamurthy Thaiya,
Hewel Johannes,
Bonzagni Neil J.,
Dabbs Jason,
Bull Robert L.,
Yates John R.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2564
Subject(s) - bacillus anthracis , chemistry , antigen , identification (biology) , monoclonal antibody , microbiology and biotechnology , anthrax toxin , bacteria , anthrax vaccines , antibody , computational biology , biology , recombinant dna , biochemistry , gene , fusion protein , immunology , genetics , botany , dna vaccination
Specific identification of Bacillus anthracis ( B. anthracis ) is vital for the accurate treatment of afflicted personnel during biological warfare situations and civilian terrorist attacks. In order to accomplish this, we have subjected the lysates from B. anthracis to affinity purification using monoclonal antibodies for the selected antigenic protein present in the bacteria. The bound antigenic protein was identified by multi‐dimensional protein identification technology (MudPIT) to be a surface layer protein EA1. The same antigen was identified from the lysates from a few strains of B. anthracis demonstrating the observation to be common for B. anthracis strains. Hence, this presents an effective pathway for the identification of the bacteria present in unknown samples of various origins. Generation of a database containing the EA1 protein has been found to be useful in the database search of unknown samples. Published in 2006 by John Wiley & Sons, Ltd.