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Identification of catecholamines in the immune system by desorption/ionization on silicon
Author(s) -
Kraj Agnieszka,
Jarzebinska Justyna,
GoreckaDrzazga Anna,
Dziuban Jan,
Silberring Jerzy
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2546
Subject(s) - chemistry , desorption , identification (biology) , ionization , silicon , immune system , chromatography , organic chemistry , ion , immunology , adsorption , botany , biology
Desorption/ionization from porous silicon dioxide (DIOSD), in combination with a standard matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) instrument, was used for the identification of catecholamines in the human peripheral blood lymphocytes. A routine MALDI‐TOF analysis does not allow for sensitive detection of low molecular mass compounds (i.e. below 400 Da) due to the pronounced background ions arising from the matrix. Therefore, we have tested DIOSD methodology for the identification of catecholamines in the immune system. Using DIOSD, catecholamines were unambiguously identified in the cell extract of peripheral blood lymphocytes at the femtomolar level. The DIOSD extends the possible use of MALDI‐TOF mass spectrometry towards small molecules that were previously detected by other methods. Copyright © 2006 John Wiley & Sons, Ltd.