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Identification of isocephalomannine in the presence of cephalomannine isomers and alkali metal ion adducts in a paclitaxel active pharmaceutical ingredient using electrospray tandem mass spectrometry
Author(s) -
Vivekanandan Kannan,
Swamy Madugundu Guru,
Prasad Sudhanand,
Mukherjee Rama,
Burman Anand C.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2500
Subject(s) - chemistry , tandem mass spectrometry , adduct , alkali metal , ingredient , electrospray , electrospray mass spectrometry , mass spectrometry , electrospray ionization , chromatography , stereochemistry , organic chemistry , food science
A strategy is developed for the identification of isocephalomannine in the presence of alkali metal ion adducts and other cephalomannine isomers in a paclitaxel active pharmaceutical ingredient. Intact molecular ion analyses and a sub‐structural study have been performed for the differentiation of isocephalomannine (2‐debenzoylpaclitaxel‐2‐pentenoate) from cephalomannine and 7‐epi‐cephalomannine. A comparative study of the cephalomannine isomers was carried out using molecular ions (MS) and fragmentation patterns (MS/MS) for sub‐structural analysis. An attempt has been made to identify isocephalomannine in Cremophor® EL formulations. Copyright © 2006 John Wiley & Sons, Ltd.