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Simultaneous quantification of CTN986 and its deglycosylation products in rat serum using liquid chromatography/tandem mass spectrometry
Author(s) -
Guo Jifen,
Zhao Yimin,
Zhao Ling,
Zhang Weiqiang,
Zhang Aijun,
Xu Bin
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2491
Subject(s) - chemistry , chromatography , formic acid , selected reaction monitoring , tandem mass spectrometry , rutin , analyte , mass spectrometry , liquid chromatography–mass spectrometry , extraction (chemistry) , electrospray ionization , solid phase extraction , high performance liquid chromatography , pharmacokinetics , quantitative analysis (chemistry) , protein precipitation , methanol , biochemistry , medicine , antioxidant , organic chemistry
A quantitative method for the simultaneous determination of CTN986, a flavonol triglycoside, and its two deglycosylation products rutin and hirsutin in rat serum was developed and validated for the investigation of the pharmacokinetics of CTN986. Analytes were isolated from the serum samples (200 µL) prior to analysis by liquid chromatography/tandem mass spectrometry (LC/MS/MS) using C 18 solid‐phase extraction, and were separated on a Zorbax C 8 reversed‐phase column with an isocratic mobile phase consisting of methanol/isopropanol/water/formic acid (20:10:70:0.1, v/v/v/v). The protonated analytes generated in the positive ion mode were monitored through multiple reaction monitoring in an eletrospray ionization source. Calibration was performed by internal standardization with CTN987, a flavonoid structurally similar to CTN986, and regression curves were constructed ranging from 2 to 1000 ng/mL in 200 µL serum samples. The intra‐ and inter‐day precision values were below 11% and accuracy was between −2.37 and 1.4% for all quality control samples. This quantitation method was successfully applied to pharmacokinetic studies of CTN986 in rats following oral and intravenous administration. Rutin and hirsutin were not detected in rat serum. Copyright © 2006 John Wiley & Sons, Ltd.