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Enhanced detection of sulfo‐peptides as onium salts in matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Ueki Masaaki,
Yamaguchi Miyuki
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2480
Subject(s) - chemistry , phosphonium , mass spectrometry , adduct , bromide , onium , time of flight mass spectrometry , matrix assisted laser desorption/ionization , desorption , hexafluorophosphate , chromatography , ionization , ion , organic chemistry , ionic liquid , catalysis , adsorption
A new two‐component system, consisting of a matrix and an onium salt as comatrix, is described for detection of sulfo‐peptides in the positive mode by matrix‐assisted desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS). Binary iodonium salts were superior to quaternary phosphonium salts in terms of suppression of desulfation and salt formation with the carboxyl group. Of the iodonium salts examined, bis(4‐ tert ‐butylphenyl)iodonium (BTI) hexafluorophosphate and bromide were most effective in giving intensive molecular ion signals in the form of [M(BTI)+BTI] + . The conditions optimized for O ‐sulfated tyrosine‐containing peptides could be applicable for O ‐sulfated serine‐ and threonine‐containing peptides. In the case of a phospho‐peptide, a molecular ion appeared more intensively as a proton adduct than as a BTI adduct. Copyright © 2006 John Wiley & Sons, Ltd.