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Analysis of strychnos alkaloids using electrospray ionization Fourier transform ion cyclotron resonance multi‐stage tandem mass spectrometry
Author(s) -
Yan Jing,
Liu Zhiqiang,
Yan Cunyu,
Xing Junpeng,
Liu Shuying
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2455
Subject(s) - chemistry , fourier transform ion cyclotron resonance , fragmentation (computing) , tandem mass spectrometry , mass spectrometry , electrospray ionization , top down proteomics , strychnos , ion cyclotron resonance , analytical chemistry (journal) , selected reaction monitoring , protein mass spectrometry , ion , chromatography , alkaloid , organic chemistry , cyclotron , computer science , operating system
The fragmentations of four strychnos alkaloids have been investigated by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI‐FTICR‐MS) in the positive ion mode. Experiments using multi‐stage tandem mass spectrometry (ESI‐FT‐ICR‐MS n ) allowed us to obtain precise elemental compositions of product ions at high mass resolution. The experimental data demonstrated that the nitrogen bridge and the coordinated oxygen atom on the nitrogen bridge in the alkaloid compounds were the active sites in the MS 2 fragmentations. The loss of CH 3 or the OCH 3 group in those alkaloids, which have an OCH 3 substituent, was the dominant fragmentation mode in the MS 3 fragmentations. Logical fragmentation schemes for strychnos alkaloids have been proposed and these should be useful for the identification of these compounds. Copyright © 2006 John Wiley & Sons, Ltd.