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Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow‐injection electrospray ionization mass spectrometry
Author(s) -
Favretto Donata,
Piovan Anna,
Filippini Raffaella,
Caniato Rosamaria
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.239
Subject(s) - catharanthus roseus , chemistry , vinblastine , electrospray ionization , vincristine , somatic embryogenesis , mass spectrometry , chromatography , electrospray , tissue culture , biochemistry , chemotherapy , in vitro , biology , cyclophosphamide , genetics
A semiquantitative determination of two bis‐indole antitumor alkaloids, vincristine and vinblastine, has been performed by flow‐injection electrospray ionization mass spectrometric analysis of the extracts of Catharanthus roseus . Leaves and flowers of two different phenotypes (pink flower and white flower) obtained from somatic embryogenesis were thus examined and compared with the field‐grown mother plant. Different amounts of vincristine and vinblastine were detected depending on the examined samples. Copyright © 2001 John Wiley & Sons, Ltd.