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UV photodissociation of phospho‐seryl‐containing peptides: laser stabilization of the phospho‐seryl bond with multistage mass spectrometry
Author(s) -
Lemoine Jérôme,
Tabarin Thibault,
Antoine Rodolphe,
Broyer Michel,
Dugourd Philippe
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2333
Subject(s) - chemistry , photodissociation , fragmentation (computing) , homolysis , dissociation (chemistry) , mass spectrometry , phosphate , protonation , photochemistry , ion , bond cleavage , collision induced dissociation , tandem mass spectrometry , stereochemistry , radical , chromatography , organic chemistry , catalysis , computer science , operating system
Protonated precursor ions of phosphorylated peptides containing a tyrosyl residue have been subjected to UV laser‐induced dissociation (LID) at a wavelength of 220 nm and to collision‐induced dissociation (CID) in an ion trap. As expected, neutral loss of the phosphate group is one of the predominant fragmentation channels during CID together with H 2 O elimination. In contrast, LID leads mainly to the homolytic cleavage of the tyrosyl side chain and a restrained loss of the phosphate group. Interestingly, the intensity of the dephosphorylated fragment ion is greatly minimized when CID is carried out next on the radical precursor ion of the singly and doubly charged species. Copyright © 2006 John Wiley & Sons, Ltd.