Premium
Detection of benzopyrene‐deoxyguanosine adducts by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Qiao Zhuanhong,
Udeochu Uche,
Jimerson Toiya,
Fletcher Melissa,
Bakare Oladapo,
Hosten Charles M.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2328
Subject(s) - chemistry , mass spectrometry , adduct , guanosine , deoxyguanosine , chromatography , matrix assisted laser desorption/ionization , benzopyrene , desorption , ionization , sample preparation in mass spectrometry , surface enhanced laser desorption/ionization , sample preparation , analytical chemistry (journal) , electrospray ionization , pyrene , benzo(a)pyrene , organic chemistry , biochemistry , ion , adsorption
A method for the detection of BPDE‐d guanosine adducts using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) is described and illustrated. The results indicate that MALDI is capable of detecting two other DNA benzopyrene adducts, which are trace products formed during the synthesis of BPDE‐d guanosine. This MALDI‐TOFMS method offers the potential for the detection of DNA adducts in human tissue using very limited sample purification and preparation. Copyright © 2006 John Wiley & Sons, Ltd.