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Atmospheric pressure ionisation mass spectrometric fragmentation pathways of noscapine and papaverine revealed by multistage mass spectrometry and in‐source deuterium labelling
Author(s) -
Wickens James R.,
Sleeman Richard,
Keely Brendan J.
Publication year - 2006
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2325
Subject(s) - chemistry , noscapine , mass spectrometry , fragmentation (computing) , papaverine , sanguinarine , ion , chromatography , analytical chemistry (journal) , tandem mass spectrometry , ion trap , collision induced dissociation , electrospray , selected reaction monitoring , deuterium , alkaloid , stereochemistry , organic chemistry , quantum mechanics , medicine , physics , computer science , operating system
Two opium alkaloids, noscapine and papaverine, show good response as [M+H] + ions in positive ion electrospray mass spectrometry and atmospheric pressure chemical ionisation mass spectrometry. The two compounds exhibit markedly different fragmentation pathways and behaviour under multistage mass spectrometry (MS n ), with papaverine displaying a wealth of ions in MS 2 and noscapine providing a single dominant ion at each stage of MS n prior to MS 4 . Elucidation of the fragmentation pathways using the MS n capability of the ion trap was aided by spraying the analytes in 2 H 2 O to incorporate an isotopic label. Simplex optimisation allowed optimum trapping and fragmentation parameters to be determined, leading to a six‐fold improvement in response for one transition and a seven‐fold improvement for one transition sequence. Copyright © 2006 John Wiley & Sons, Ltd.