Premium
A new hybrid electrospray Fourier transform mass spectrometer: design and performance characteristics
Author(s) -
O'Connor Peter B.,
Pittman Jason L.,
Thomson Bruce A.,
Budnik Bogdan A.,
Cournoyer Jason C.,
Jebanathirajah Judith,
Lin Cheng,
Moyer Susanne,
Zhao Cheng
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2307
Subject(s) - chemistry , fourier transform ion cyclotron resonance , mass spectrometry , electrospray , ion trap , analytical chemistry (journal) , quadrupole ion trap , ion , hybrid mass spectrometer , quadrupole , electrospray ionization , ion cyclotron resonance , tandem mass spectrometry , atomic physics , selected reaction monitoring , cyclotron , chromatography , physics , organic chemistry
A new hybrid electrospray quadrupole Fourier transform mass spectrometry (FTMS) instrument design is shown and characterized. This instrument involves coupling an electrospray source and mass‐resolving quadrupole, ion accumulation, and collision cell linear ion trap system developed by MDS Sciex with a home‐built ion guide and ion cyclotron resonance (ICR) cell. The iterative progression of this design is shown. The final design involves a set of hexapole ion guides to transfer the ions from the accumulation/collision trap through the magnetic field gradient and into the cell. These hexapole ion guides are separated by a thin gate valve and two conduction limits to maintain the required <10 −9 mbar vacuum for FTICR. Low‐attomole detection limits for a pure peptide are shown, 220 000 resolving power in broadband mode and 820 000 resolving power in narrow‐band mode are demonstrated, and mass accuracy in the <2 ppm range is routinely available provided the signal is abundant, cleanly resolved, and internally calibrated. This instrument design provides high experimental flexibility, allowing Q2 CAD, SORI‐CAD, IRMPD, and ECD experiments with selected ion accumulation as well as experiments such as nozzle skimmer dissociation. Initial top‐down mass spectrometry experiments on a protein is shown using ECD. Copyright © 2005 John Wiley & Sons, Ltd.