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Enantiomeric separation and quantification of pindolol in human plasma by chiral liquid chromatography/tandem mass spectrometry using staggered injection with a CTC Trio Valve system
Author(s) -
Wang Haiping,
Shen Zhongzhou
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2293
Subject(s) - pindolol , chemistry , chromatography , enantiomer , tandem mass spectrometry , mass spectrometry , electrospray ionization , liquid chromatography–mass spectrometry , tandem , stereochemistry , biochemistry , materials science , receptor , composite material
Pindolol is a non‐selective β ‐adrenergic antagonist ( β ‐blocker) for the treatment of cardiovascular diseases such as hypertension and angina pectoris. It has one chiral center, and, therefore, two optical isomers. It was essential to develop an enantioselective assay to measure each enantiomer in human plasma. However, separation of enantiomers using chiral chromatography usually requires relatively long retention times. This can pose a problem for rapid turnaround of a large number of samples (i.e., clinical studies). In the present study, a simple and sensitive chiral liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) method was developed and validated for the determination of S ‐(−)‐ and R ‐(+)‐pindolol in human plasma. To increase throughput, staggered sample injection was employed using a CTC Trio Valve system on a CTC HTS PAL autosampler. The method exhibited good intra‐ and inter‐day accuracy and precision, and was linear over a dynamic range of 250 pg/mL to 250 ng/mL for each pindolol enantiomer. Intra‐ and inter‐day accuracy ranged between 90.0–106% and 91.6–104% for both quality control (QC) samples of S ‐(−)‐ and R ‐(+)‐pindolol, respectively. The respective intra‐ and inter‐day precision ranged between 4.24–7.86% and 4.98–10.4%. Copyright © 2005 John Wiley & Sons, Ltd.