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Characterization of boldione and its metabolites in human urine by liquid chromatography/electrospray ionization mass spectrometry and gas chromatography/mass spectrometry
Author(s) -
Kim Yunje,
Jun Myungyoon,
Lee Won
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2271
Subject(s) - chemistry , chromatography , urine , mass spectrometry , derivatization , electrospray ionization , gas chromatography–mass spectrometry , gas chromatography , biochemistry
Boldione (1,4‐androstadiene‐3,17‐dione) is a direct precursor (prohormone) to the anabolic steroid boldenone (1,4‐androstadiene‐17β‐ol‐3‐one). It is advertised as a highly anabolic/androgenic compound promoting muscularity, enhancing strength and overall physical performance, and is available on the Internet and in health stores. This work was undertaken to determine and characterize boldione and its metabolites in human urine, using both liquid chromatography with electrospray ionization mass spectrometry and gas chromatography with mass spectrometry and derivatization. Boldione and its three metabolites were detected in dosed human urine after dosing a healthy volunteer with 100 mg boldione. The excretion studies showed that boldione and its metabolites were detectable in urine for 48 h after oral administration, with maximum excretion rates after 1.8 and 3.6 h (boldenone case). The amounts of boldione and boldenone excreted in urine from this 100 mg dose were 34.45 and 15.95 mg, respectively. Copyright © 2005 John Wiley & Sons, Ltd.