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Detection limits of matrix‐assisted laser desorption/ionisation mass spectrometry coupled to chromatography – a new application of solvent‐free sample preparation
Author(s) -
Falkenhagen Jana,
Weidner Steffen M.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2256
Subject(s) - chemistry , mass spectrometry , analyte , matrix (chemical analysis) , chromatography , sample preparation , analytical chemistry (journal) , matrix assisted laser desorption/ionization , maldi imaging , desorption , reproducibility , solvent , organic chemistry , adsorption
The detection limits of matrix‐assisted laser desorption/ionisation mass spectrometry (MALDI‐MS) by semi‐online coupling with chromatography were investigated using various mixtures of polyethylene oxides (PEOs) with different end groups. In contrast to the common ‘dried‐droplet’ sample preparation technique, which results in an inhomogeneous sample‐to‐matrix ratio within the MALDI spot, the used coupling technique offers a very high reproducibility combined with surpassing sensitivity of a few femtograms over a broad range of sample‐to‐matrix ratios. These results are in correlation with the results of the recently established solvent‐free MALDI‐TOFMS method utilising the grinding approach and are also of assistance towards the more theoretical aspect of MALDI that suggests that there is no necessity for an analyte incorporation into a matrix crystal for excellent matrix‐assistance. Copyright © 2005 John Wiley & Sons, Ltd.