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Determination of 4‐hydroxytamoxifen in mouse plasma in the pg/mL range by gradient capillary liquid chromatography/tandem mass spectrometry
Author(s) -
Plumb R. S.,
Warwick H.,
Higton D.,
Dear G. J.,
Mallett D. N.
Publication year - 2001
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.225
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , tandem , capillary action , detection limit , high performance liquid chromatography , liquid chromatography–mass spectrometry , plasma , analytical chemistry (journal) , materials science , physics , quantum mechanics , composite material
Capillary high‐performance liquid chromatography (HPLC; 300 µm i.d.) coupled to tandem mass spectrometry has been used to determine the concentration of 4‐hydroxytamoxifen in mouse plasma in the pg/mL range following the administration of Tamoxifen. A limit of quantification (LOQ) of 100 pg/mL was achieved using only 25 µL of plasma. The on‐column sensitivity was determined to be 100 fg. The column performance was determined isocratically before and after the assay and showed only a 15% reduction in performance after 70 injections of plasma extract. No significant peak band broadening was observed due to the mass spectrometer interface using a standard TurboIonspray source. Copyright © 2001 John Wiley & Sons, Ltd.