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High sensitivity determination of valproic acid in mouse plasma using semi‐automated sample preparation and liquid chromatography with tandem mass spectrometric detection
Author(s) -
Pucci Vincenzo,
Monteagudo Edith,
Bonelli Fabio
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2249
Subject(s) - chemistry , chromatography , tandem , liquid chromatography–mass spectrometry , tandem mass spectrometry , valproic acid , plasma , mass spectrometry , epilepsy , materials science , physics , quantum mechanics , neuroscience , composite material , biology
A high‐throughput liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) assay using automated sample preparation has been developed for the determination of valproic acid (VPA) in mouse plasma. A liquid‐handling system was programmed to prepare calibration standard solutions in plasma, as well as quality controls and clinical samples. Plasma protein precipitation was performed on a 96‐well plate, and the collected supernatant was directly injected into a reversed‐phase LC/ESI‐MS/MS system in the negative ionization mode. The calibration curve for VPA was linear over a dynamic range of 0.15–100 µg/mL. The limit of detection was 75 ng/mL and the lower limit of quantitation was 150 ng/mL. Intra‐ and inter‐day validation assays of the semi‐automated plasma analysis showed satisfactory accuracy and precision. Copyright © 2005 John Wiley & Sons, Ltd.