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Use of electrospray ionization mass spectrometry for profiling of crude oil effects on the phospholipid molecular species of two marine bacteria
Author(s) -
Mazzella Nicolas,
Molinet Josiane,
Syakti Agung Dhamar,
Dodi Alain,
Bertrand JeanClaude,
Doumenq Pierre
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2231
Subject(s) - chemistry , phospholipid , electrospray ionization , chromatography , mass spectrometry , bacteria , membrane , fatty acid , lipidomics , electrospray , biochemistry , biology , genetics
We investigated the membrane lipid composition of two hydrocarbon‐degrading gram‐negative bacterial strains ( Pseudomonas nautica IP 617 and Marinobacter hydrocarbonoclasticus ) initially cultured on a soluble substrate, then on petroleum hydrocarbons, and finally taken back onto the soluble substrate. For the two strains, the growth on petroleum and the return to the initial medium showed major, but comparable, qualitative and quantitative modifications of the intact phospholipid molecular species (IPMS) composition. Furthermore, since bacterial membranes are mainly made up of phospholipids, these modifications reflected hydrocarbon assimilation, restoration abilities and membrane fluidity adaptation. The electrospray ionization mass spectrometry (ESI‐MS) analysis of intact phospholipid provided some new information (e.g. sn fatty acyl chain distribution) that could not be assessed by the classical fatty acid analysis. Moreover, such information should be particularly helpful with regards to bacterial taxonomy and xenobiotic toxicity studies. Copyright © 2005 John Wiley & Sons, Ltd.