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Direct infusion mass spectrometry as a fingerprint of protein‐binding media used in works of art
Author(s) -
PerisVicente J.,
SimóAlfonso E.,
Gimeno Adelantado J. V.,
Doménech Carbó M. T.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2217
Subject(s) - chemistry , chromatography , derivatization , mass spectrometry , electrospray , casein , amino acid , hydrolysis , acid hydrolysis , bovine serum albumin , yield (engineering) , organic chemistry , biochemistry , materials science , metallurgy
A direct infusion mass spectrometry method for the characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Amino acids are released by acid hydrolysis and dissolved in a mixture of acidic water and ethanol. This mixture is directly infused into a mass spectrometer without any derivatization. The mass spectrometer is operated in positive ion electrospray mode (ESI‐MS) to yield [M+H] + ions for the amino acids. Relative amounts of each amino acid are calculated for each protein (beef and porcine gelatines, albumin, casein and egg). The analyzed proteins were satisfactorily distinguished. The method is easy and fast, and shows good sensitivity and resolution. The proposed method has been successfully applied to artistic samples from items of the cultural heritage of Valencia (Spain). Copyright © 2005 John Wiley & Sons, Ltd.