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Determination of microbial volatile organic compounds from Staphylococcus pasteuri against Tuber borchii using solid‐phase microextraction and gas chromatography/ion trap mass spectrometry
Author(s) -
Barbieri E.,
Gioacchini A. M.,
Zambonelli A.,
Bertini L.,
Stocchi V.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2209
Subject(s) - chemistry , solid phase microextraction , truffle , mycelium , mass spectrometry , gas chromatography–mass spectrometry , chromatography , gas chromatography , food science , botany , biology
The mycelium of Tuber borchii Vittad., a commercial truffle species, is used as a model system for in vitro ectomycorrhizal synthesis, infected seedling production and biotechnological applications. Our fungal cultures were accidentally contaminated with a Staphylococcus pasteuri strain, showing a strong antifungal activity against T. borchii mycelium. In order to identify the antifungal volatile agents produced by S. pasteuri , solid‐phase microextraction (SPME) with gas chromatography and mass spectrometry (GC/MS) was used. Using this method 65 microbial volatile organic compounds (MVOCs), synthesized by this bacterium in either single or in fungal‐bacterial dual culture, were identified. SPME combined with GC/MS may be a useful method for the determination of MVOCs involved in the antifungal activity. These results showed that bacteria with unusual biological activities could be a major problem during large‐scale production of inoculum for truffle‐infected seedling. Copyright © 2005 John Wiley & Sons, Ltd.