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In‐house validation of a liquid chromatography/electrospray tandem mass spectrometry method for confirmation of chloramphenicol residues in muscle according to Decision 2002/657/EC
Author(s) -
Vinci Floriana,
Guadagnuolo Grazia,
Danese Vincenzo,
Salini Marco,
Serpe Luigi,
Gallo Pasquale
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2200
Subject(s) - chemistry , chromatography , electrospray , liquid chromatography–mass spectrometry , tandem mass spectrometry , mass spectrometry , veterinary drug , electrospray ionization , european commission , european union , business , economic policy
In this work we present an in‐house validation study for the confirmatory analysis of chloramphenicol (CAP) in muscle according to the Commission Decision 2002/657/EC requirements. CAP is extracted in acetonitrile and after liquid‐liquid partitioning with n ‐hexane is identified and quantitatively determined by ion trap liquid chromatography/electrospray ionisation tandem mass spectrometry (LC/ESI‐MS/MS) analysis in the negative ion mode. CAP was identified using the precursor ion and at least two product ions, meeting the qualitative and quantitative criteria set by the European Commission in the Decision 2002/657/EC for confirmation of prohibited veterinary drug residues. We calculated mean drug recoveries, CC α and CC β of the method, and reported data on specificity, ruggedness and within‐laboratory reproducibility. Finally, we point out and discuss some problems and questions arising from controversy about the application of Decision 2002/657/EC. Copyright © 2005 John Wiley & Sons, Ltd.