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A new reversed‐phase liquid chromatographic/tandem mass spectrometric method for analysis of underivatised amino acids: evaluation for the diagnosis and the management of inherited disorders of amino acid metabolism
Author(s) -
Piraud M.,
VianeySaban C.,
Bourdin C.,
AcquavivaBourdain C.,
Boyer S.,
Elfakir C.,
Bouchu D.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2197
Subject(s) - chemistry , chromatography , analyte , quantitative analysis (chemistry) , tandem mass spectrometry , electrospray , amino acid , mass spectrometry , electrospray ionization , isotope dilution , elution , ion suppression in liquid chromatography–mass spectrometry , gradient elution , high performance liquid chromatography , liquid chromatography–mass spectrometry , tandem , acetonitrile , reversed phase chromatography , biochemistry , materials science , composite material
Seventy‐six compounds of biological interest for the diagnosis of inherited disorders of amino acids (AA) metabolism have previously been demonstrated to be detectable in positive mode electrospray ionisation tandem mass spectrometry (ESI‐MS/MS), after separation by ion‐pairing reversed‐phase liquid chromatography (RPLC). The separation method used tridecafluoroheptanoic acid as ion‐pairing agent, and a gradient of acetonitrile for the elution of the most retained compounds. This method had previously been demonstrated to be suitable for the qualitative diagnosis of many AA disorders, and for the quantitative measurement of 16 AA in biological fluids, using their stable isotope labelled (SIL) AA as internal standard. For quantification of the other AA, an internal standard was chosen among the available SIL‐AA, as close as possible to the analyte to be measured, in terms of structural analogy, and of retention time in the chromatographic system. The performances of the quantitative analysis of the other AA to be measured are reported here. They show validated results for several AA, allowing their accurate quantification, with another SIL‐AA as internal standard. For some other AA, quantitative results were not accurate, allowing only semi‐quantitative or qualitative determination for these parameters. Copyright © 2005 John Wiley & Sons, Ltd.