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Identification and mass spectrometric characterization of glycosylated flavonoids in Triticum durum plants by high‐performance liquid chromatography with tandem mass spectrometry
Author(s) -
Cavaliere Chiara,
Foglia Patrizia,
Pastorini Elisabetta,
Samperi Roberto,
Laganà Aldo
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2185
Subject(s) - chemistry , tricin , chromatography , luteolin , tandem mass spectrometry , mass spectrometry , apigenin , flavones , glycoside , high performance liquid chromatography , liquid chromatography–mass spectrometry , flavonoid , organic chemistry , antioxidant
A mass spectrometric method for extensive detection and semi‐quantitative determination of flavonoid glycosides in stem and leaves of young Triticum durum plants is presented. About 100 g of sample were lyophilized and ground, and the compounds of interest were then extracted, cleaned‐up, and fractionated using high‐performance liquid chromatography (HPLC). Tandem mass spectrometry analyses were performed using a quadrupole‐linear ion trap instrument with an information‐dependent data acquisition (IDA) protocol that looped two experiments, enhanced MS scan and enhanced product ion scan. Various glycoconjugates, which are all derivatives of only four flavones, apigenin, luteolin, chrysoeriol and tricin, were identified and belong to the following categories: 7 monoglycosides, 31 diglycosides, 15 triglycosides and 1 tetraglycoside. Among these some acylated glycosides were found. Tricin derivatives are present exclusively as O ‐glycosides, while apigenin and luteolin are present always as C ‐glycosides. Semi‐quantitative estimation was performed by using the monoglycoside and diglycoside of quercetin as internal standards. Copyright © 2005 John Wiley & Sons, Ltd.