Premium
Characterization of a new methylated β ‐cyclodextrin with a low degree of substitution by electrospray ionization mass spectrometry and liquid chromatography/mass spectrometry
Author(s) -
Jacquet Romain,
Elfakir Claire,
Lafosse Michel
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2175
Subject(s) - chemistry , electrospray ionization , mass spectrometry , chromatography , electrospray , fragmentation (computing) , liquid chromatography–mass spectrometry , ionization , electrospray mass spectrometry , analytical chemistry (journal) , cyclodextrin , organic chemistry , ion , computer science , operating system
A new methylated β ‐cyclodextrin (Me‐ β ‐CD) with a low degree of substitution (DS) was characterized by electrospray ionization mass spectrometry (ESI‐MS) and liquid chromatography coupled with ESI‐MS (LC/ESI‐MS). For ESI‐MS analyses, the composition of the infused sample solution was optimized in order to obtain only singly charged ammoniated CDs without fragmentation. The DS value (i.e. the number of methyl groups per glucopyranose unit) was found to be 0.7, which was in accordance with the values previously obtained by other methods. The LC/ESI‐MS analysis, derived from a method using evaporative light scattering detection, allowed the study of the substitution isomers of each derivative and appears to be an easy and rapid tool for the accurate characterization of Me‐ β ‐CD mixtures. Copyright © 2005 John Wiley & Sons, Ltd.