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Screening for proteolytic activities in snake venom by means of a multiplexing electrospray ionization mass spectrometry assay scheme
Author(s) -
Liesener André,
Perchuc AnnaMaria,
Schöni Reto,
Wilmer Marianne,
Karst Uwe
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2136
Subject(s) - chemistry , chromatography , proteases , electrospray ionization , proteolytic enzymes , venom , mass spectrometry , substrate (aquarium) , bothrops , enzyme , biochemistry , snake venom , oceanography , geology
A multiplexed mass spectrometry based assay scheme for the simultaneous determination of five different substrate/product pairs was developed as a tool for screening of proteolytic activities in snake venom fractions from Bothrops moojeni . The assay scheme was employed in the functional characterization of eight model proteases. Time‐resolved reaction profiles were generated and the relative reaction progress at each time point was determined. These were used to semi‐quantitatively sort the catalytic activities of each enzyme towards the respective substrates into six classes. The resulting activity pattern served as an activity fingerprint for each enzyme. The multiplex assay scheme was then applied to a screening for proteolytic activities in fractions of the pre‐separated venom from B. moojeni . Activity patterns of each fraction were generated and used to sort the fractions into three different categories of activity. By comparison of the fingerprint activity patterns of the venom fractions and the model enzymes, a compound with proteolytic properties similar to activated protein C was detected. Copyright © 2005 John Wiley & Sons, Ltd.