Premium
On‐line pretreatment using methylcellulose‐immobilized cation‐exchange restricted access media for direct liquid chromatography/mass spectrometric determination of basic drugs in plasma
Author(s) -
Kawano Shinichi,
Takahashi Masatoshi,
Hine Takashi,
Yamamoto Eiichi,
Asakawa Naoki
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2130
Subject(s) - chemistry , chromatography , plasma , line (geometry) , analytical chemistry (journal) , physics , geometry , mathematics , quantum mechanics
Abstract A novel methylcellulose‐immobilized restricted access media column with strong cation‐exchange groups on an internal surface (MC‐SCX) was evaluated for the direct injection analysis of basic polar drugs in plasma by column‐switching liquid chromatography/mass spectrometry (LC/MS). Analytical conditions, including an automated pretreatment step and MS detection, were optimized for a series of basic drugs (doxepin, desipramine, imipramine, nortriptyline, amitriptyline, clomipramine). On‐line pretreatment with the MC‐SCX column followed by fast gradient analysis using a C18 column resulted in a total analysis cycle time of 7 min for each spiked plasma sample. More than 150 plasma samples spiked with target compounds were measured without compromising MS detection (relative standard deviations less than 11% for all compounds, and regression coefficients greater than 0.99). Copyright © 2005 John Wiley & Sons, Ltd.