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Determination of seven benzoylphenylurea insecticides in processed fruit and vegetables using high‐performance liquid chromatography/tandem mass spectrometry
Author(s) -
Sannino Anna,
Bandini Mirella
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2108
Subject(s) - chemistry , chromatography , mass spectrometry , tandem mass spectrometry , diflubenzuron , electrospray ionization , electrospray , elution , selected reaction monitoring , extraction (chemistry) , pesticide , agronomy , biology
Abstract A liquid chromatographic method with electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) has been developed for the sensitive and selective determination of seven benzoylphenylurea insecticide residues (diflubenzuron, triflumuron, lufenuron, flufenoxuron, teflubenzuron, chlorfuazuron, hexaflumuron) in pear baby purée, concentrated lemon juice, and tomato pulp. The general sample extraction/partition method for our established multiresidue methods has been used. The entire procedure involves extraction of residues with acetone and partition into ethyl acetate/cyclohexane. Chromatographic determination was performed using a C18 column and isocratic elution. Fourteen MS/MS transitions of precursor ions were monitored (two for each pesticide) using negative ESI. The majority of mean recoveries at fortification levels of 0.002–0.020 and 0.020–0.200 mg/kg were in the range 77–102% with relative standard deviations between 2 and 10%. The excellent sensitivity and selectivity of this LC/MS/MS method allowed quantitation and identification at low levels in difficult matrices with a run time of 4 min. Copyright © 2005 John Wiley & Sons, Ltd.