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Simultaneous detection of two bacterial pathogens using bacteriophage amplification coupled with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry
Author(s) -
Rees Jon C.,
Voorhees Kent J.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2107
Subject(s) - chemistry , mass spectrometry , ionization , matrix (chemical analysis) , matrix assisted laser desorption/ionization , laser , desorption , surface enhanced laser desorption/ionization , time of flight mass spectrometry , chromatography , analytical chemistry (journal) , sample preparation in mass spectrometry , electrospray ionization , optics , ion , organic chemistry , adsorption , physics
The simultaneous analysis of multiple target microorganisms using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) with bacteriophage amplification is discussed. Following infection of target bacteria with specific bacteriophages, proteins contained in the progeny phage are utilized as a secondary biomarker for the target bacterium. Escherichia coli when mixed with MS2 and MPSS‐1 phages specific for E. coli and Salmonella spp., respectively, at levels below their corresponding detection limits, produced only the protein (13.7 kDa) characteristic of the MS2. Likewise, Salmonella spp. when mixed with the two phages only produced a protein (13.5 kDa) characteristic of MPSS‐1. When the two bacteria and the two phages were mixed together, proteins characteristic of MS2 and MPSS‐1 were observed indicating that both bacteriophages had been amplified. Identification of each bacterium was made based on the presence of the secondary bacteriophage biomarkers. No deleterious effects on bacteriophage amplification were observed because of the presence of multiple bacteria or bacteriophages. Copyright © 2005 John Wiley & Sons, Ltd.