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Identification of phospholipid structures in human blood by direct‐injection quadrupole‐linear ion‐trap mass spectrometry
Author(s) -
Wang Chang,
Yang Jun,
Gao Peng,
Lu Xin,
Xu Guowang
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2080
Subject(s) - chemistry , phospholipid , sphingomyelin , phosphatidylethanolamine , phosphatidylserine , quadrupole ion trap , mass spectrometry , chromatography , electrospray ionization , lipidomics , phosphatidylcholine , ion trap , electrospray , ion , analytical chemistry (journal) , tandem mass spectrometry , membrane , biochemistry , organic chemistry
Direct‐injection electrospray ionization mass spectrometry in combination with information‐dependent data acquisition (IDA), using a triple‐quadrupole/linear ion trap combination, allows high‐throughput qualitative analysis of complex phospholipid species from child whole blood. In the IDA experiments, scans to detect specific head groups (precursor ion or neutral loss scans) were used as survey scans to detect phospholipid classes. An enhanced resolution scan was then used to confirm the mass assignments, and the enhanced product ion scan was implemented as a dependent scan to determine the composition of each phospholipid class. These survey and dependent scans were performed sequentially and repeated for the entire duration of analysis, thus providing the maximum information from a single injection. In this way, 50 different phospholipids belonging to the phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylcholine and sphingomyelin classes were identified in child whole blood. Copyright © 2005 John Wiley & Sons, Ltd.