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Self‐aspirating atmospheric pressure chemical ionization source for direct sampling of analytes on surfaces and in liquid solutions
Author(s) -
Asano Keiji G.,
Ford Michael J.,
Tomkins Bruce A.,
Van Berkel Gary J.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2062
Subject(s) - chemistry , atmospheric pressure chemical ionization , chromatography , analyte , analytical chemistry (journal) , mass spectrometry , ion trap , chemical ionization , ambient ionization , solvent , sample preparation , ionization , ion , organic chemistry
A self‐aspirating heated nebulizer probe is described and demonstrated for use in the direct analysis of analytes on surfaces and in liquid samples by atmospheric pressure chemical ionization (APCI) mass spectrometry. Functionality and performance of the probe as a self‐aspirating APCI source is demonstrated using reserpine and progesterone as test compounds. The utility of the probe to sample analytes directly from surfaces was demonstrated first by scanning development lanes of a reversed‐phase thin‐layer chromatography plate in which a three‐component dye mixture, viz., Fat Red 7B, Solvent Green 3, and Solvent Blue 35, was spotted and the components were separated. Development lanes were scanned by the sampling probe operated under computer control (x, y plane) while full‐scan mass spectra were recorded using a quadrupole ion trap mass spectrometer. In addition, the ability to sample the surface of pharmaceutical tablets (viz., Extra Strength Tylenol® and Evista® tablets) and to detect the active ingredients (acetaminophen and raloxifene, respectively) selectively was demonstrated using tandem mass spectrometry (MS/MS). Finally, the capability to sample analyte solutions from the wells of a 384‐well microtiter plate and to perform quantitative analyses using MS/MS detection was illustrated with cotinine standards spiked with cotinine‐ d 3 as an internal standard. Published in 2005 by John Wiley & Sons, Ltd.