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Fragmentation of oligosaccharide ions with 157 nm vacuum ultraviolet light
Author(s) -
Devakumar Arugadoss,
Thompson Matthew S.,
Reilly James P.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2058
Subject(s) - chemistry , fragmentation (computing) , vacuum ultraviolet , ion , ultraviolet , photochemistry , optoelectronics , atomic physics , organic chemistry , computer science , operating system , physics
The 157 nm photofragmentation of native and derivatized oligosaccharides was studied in a linear ion trap and in a home‐built matrix‐assisted laser desorption/ionization (MALDI) tandem time‐of‐flight (TOF/TOF) mass spectrometer, and the results were compared with collision‐induced dissociation (CID) experiments. Photodissociation produces product ions corresponding to high‐energy fragmentation pathways; for cation‐derivatized oligosaccharides, it yields strong cross‐ring fragment ions and provides better sequence coverage than low‐ and high‐energy CID experiments. On the other hand, for native oligosaccharides, CID yielded somewhat better sequence coverage than photodissociation. The ion trap enables CID hybrid MS 3 experiments on the high‐energy fragment ions obtained from photodissociation. Copyright © 2005 John Wiley & Sons, Ltd.