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Structural characterization of 2‐aminobenzamide‐derivatized oligosaccharides using a matrix‐assisted laser desorption/ionization two‐stage time‐of‐flight tandem mass spectrometer
Author(s) -
Morelle Willy,
Slomianny MarieChristine,
Diemer Hélène,
Schaeffer Christine,
Dorsselaer Alain van,
Michalski JeanClaude
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2033
Subject(s) - chemistry , mass spectrometry , oligosaccharide , glycosidic bond , tandem mass spectrometry , fragmentation (computing) , time of flight mass spectrometry , chromatography , matrix assisted laser desorption/ionization , reductive amination , protein mass spectrometry , top down proteomics , analytical chemistry (journal) , tandem , ion , desorption , ionization , organic chemistry , materials science , adsorption , composite material , computer science , enzyme , operating system , catalysis
Oligosaccharides were derivatized by reductive amination using 2‐aminobenzamide (2‐AB) and analyzed by matrix‐assisted laser desorption/ionization two‐stage time‐of‐flight (MALDI‐TOF/TOF) tandem mass spectrometry (MS/MS) in the positive ion mode. The major signals were obtained under these conditions from the [M+Na] + ions for all 2‐AB‐derivatized oligosaccharides. A systematic study was conducted on a series of 2‐AB‐derivatized oligosaccharides to allow rationalization of the fragmentation processes. The MALDI‐TOF/TOF‐MS/MS spectra of the [M+Na] + ions of 2‐AB‐derivatized oligosaccharides were dominated by glycosidic cleavages. These fragments originating both from the reducing and the non‐reducing ends of the oligosaccharide yield information on sequence and branching. Moreover, the MALDI‐TOF/TOF‐MS/MS spectra were also characterized by abundant cross‐ring fragments which are very informative on the linkages of the monosaccharide residues constituting these oligosaccharides. MALDI‐TOF/TOF‐MS/MS analysis of 2‐AB‐derivatized oligosaccharides, by providing structural information at the low‐picomole level, appears to be a powerful tool for carbohydrate structural analysis. Copyright © 2005 John Wiley & Sons, Ltd.