Premium
Solid‐phase microextraction gas chromatography/mass spectrometry: a new method for species identification of truffles
Author(s) -
Gioacchini Anna Maria,
Menotta Michele,
Bertini Luana,
Rossi Ismaela,
Zeppa Sabrina,
Zambonelli Alessandra,
Piccoli Giovanni,
Stocchi Vilberto
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2031
Subject(s) - truffle , chemistry , solid phase microextraction , chromatography , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , mass spectrum , fraction (chemistry) , analytical chemistry (journal) , sample preparation , botany , biology
This study describes a rapid method to identify different truffle species by analysis of their volatile compound fraction using static headspace solid‐phase microextraction gas chromatography/mass spectrometry. The volatile organic compounds (VOCs) were extracted using a new 2‐cm 50/30 µm DVB/CAR/PDMS fiber placed for 10 min in the headspace of the truffle sample with the vial maintained at 20°C (in a thermostatically controlled analysis room). The mass spectra of the VOC chromatograms were represented as ‘fingerprints’ of the analysed samples. Next, stepwise factorial discriminant analysis afforded a limited number of characteristic fragment ions that allowed a classification of the truffle species studied. This new method provides an effective approach to rapid quality control and identification of truffle species by analysis of their volatile fraction. Moreover, this method offers the advantage of minimizing thermal, mechanical, and chemical modifications of the truffles, thereby reducing the risk of analytical artifacts. Copyright © 2005 John Wiley & Sons, Ltd.