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Determination of the natural abundance δ 15 N of nicotine and related alkaloids by gas chromatography/isotope ratio mass spectrometry
Author(s) -
Molinié Roland,
FerchaudRoucher Véronique,
Lebreton Jacques,
Robins Richard J.
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.2025
Subject(s) - chemistry , nornicotine , chromatography , mass spectrometry , demethylation , anabasine , gas chromatography , repeatability , gas chromatography–mass spectrometry , isotope , isotope ratio mass spectrometry , alkaloid , analytical chemistry (journal) , stereochemistry , biochemistry , gene expression , physics , quantum mechanics , gene , dna methylation
A method is described by which the natural abundance δ 15 N values of nicotine, analogues, and metabolites can be determined. The alkaloids are extracted from their biological matrix by solid‐phase extraction and analysis is conducted using isotope ratio mass spectrometry interfaced to gas chromatography. Repeatability and precision are sufficient to allow differences in the δ 15 N values of less than 1.0‰ to be satisfactorily measured, with a standard deviation routinely less than 0.5‰. The methodology has been tested by determining the changes in the δ 15 N values of nicotine, N ‐methyl‐2‐phenylpyrrolidine and their respective demethylation products, nornicotine and 2‐phenylpyrrolidine, during biotransformation by cell suspension cultures of Nicotiana species. Sufficient precision and reproducibility were obtained to allow the kinetic isotope effects associated with the demethylation reaction to be calculated. Copyright © 2005 John Wiley & Sons, Ltd.