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New conditions for matrix‐assisted laser desorption/ionization mass spectrometry of native bacterial R‐type lipopolysaccharides
Author(s) -
Sturiale Luisa,
Garozzo Domenico,
Silipo Alba,
Lanzetta Rosa,
Parrilli Michelangelo,
Molinaro Antonio
Publication year - 2005
Publication title -
rapid communications in mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.528
H-Index - 136
eISSN - 1097-0231
pISSN - 0951-4198
DOI - 10.1002/rcm.1994
Subject(s) - chemistry , matrix assisted laser desorption/ionization , moiety , mass spectrometry , desorption , ion , mass spectrum , ionization , lipid a , maldi imaging , matrix (chemical analysis) , molecule , chromatography , analytical chemistry (journal) , stereochemistry , bacteria , organic chemistry , genetics , adsorption , biology
A new sample preparation method for matrix‐assisted laser desorption/ionization (MALDI) analysis of native rough‐type lipopolysaccharides (R‐type LPSs) is presented. In our MALDI mass spectra, besides the [MH] − ions, abundant ions originating from the cleavage between the 3‐deoxy‐D‐ manno ‐oct‐2‐ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R‐type LPS with the O‐deacylated one allowed us to obtain the structure of the lipid A moiety. Copyright © 2005 John Wiley & Sons, Ltd.